[摘要] 目的 构建PDCD5基因真核表达载体，并电转染BGC823细胞，获得稳定转染细胞。 方法 设计合成PDCD5基因片段，将其插入经EcoRⅠ和XhoⅠ双酶切的pcDNA3.1+表达载体，菌落PCR和基因测序进行鉴定。重组质粒电转染BGC823细胞，G418筛选数周后PCR鉴定稳定转染细胞。 结果 重组质粒经菌落PCR测序分析表明，PDCD5基因序列准确插入预期位置，且序列正确。重组质粒成功电转染BGC823细胞，并整合入细胞基因组DNA。结论 成功构建了PDCD5基因真核表达载体，并整合进BGC823细胞基因组，为研究PDCD5基因的功能及胃癌的基因治疗提供实验基础。

    [关键词] PDCD5基因；pcDNA3.1+表达载体；电转染；BGC823细胞系

    [中图分类号] R394 [文献标识码] A [文章编号] 1673-9701(2017)06-0033-03

    [Abstract] Objective To construct eukaryotic expression vector of PDCD5 gene and transfect BGC823 cells to obtain stable transfected cells. Methods The PDCD5 gene fragment was designed and inserted into pcDNA3.1+ expression vector which was digested with EcoR Ⅰ and Xho Ⅰ. Colony PCR and sequencing were used for identification. The recombinant plasmid was electrically transfected into BGC823 cells ......