促血管生成素—2在肾细胞癌中的表达及其意义(1)
【摘要】 目的：研究促血管生成素-2(Angiopoietin-2，Ang-2)在肾细胞癌中的表达及其意义。方法：用免疫组化SP法检测50例肾细胞癌组织、配对癌旁组织以及6例正常肾组织中的Ang-2，并结合临床资料进行具体分析。结果：Ang-2在肾细胞癌组织、癌旁组织及正常肾组织中的阳性表达率分别为62.0%(31/50)、28.0%(14/50)和0(0/6)，三者比较差异有统计学意义(P<0.05)。Ang-2在Ⅰ～Ⅱ期和Ⅲ～Ⅳ期的阳性表达率分别为51.4%(19/37)和92.3%(12/13)；Ang-2在术前有血尿组和无血尿组的阳性表达率分别为85.7%(12/14)和52.8%(19/36)，两者比较差异均有统计学意义(P<0.05)。结论：Ang-2在肾细胞癌组织表达明显高于非癌组织，表明其在肾细胞癌的发生发展中起重要作用，与肾细胞癌的分期、转移有关。
【关键词】 肾细胞癌； 促血管生成素-2； 免疫组化
【Abstract】 Objective：To study the expression and significance of Ang-2 in renal cell carcinoma. Method：The expression of Ang-2 was measured by immunohistochemistry in tissue sections from 6 cases of normal renal tissues， 50 cases of RCC and their pericarcinous tissues，their correlation with clinical data features were analyzed.Result：The positive rate for expression of Ang-2 in RCC， their pericarcinous and normal renal tissue were respectively 62.0%(31/50)，28.0%(14/50) and 0(0/6)，the expression of Ang-2 in RCC was significantly higher than those in pericarcinous tissue and normal renal tissue，the difference was statistically significant among them(P<0.05).The positive expression rates of Ang-2 in the stages of Ⅰ-Ⅱ and Ⅲ-Ⅳ were respectively 51.4%(19/37) and 92.3%(12/13)，the positive expression rates of Ang-2 in the stages of Ⅲ-Ⅳ was higher than those in the stages of Ⅰ-Ⅱ；the positive expression rates of Ang-2 in patients with and without hematuresis were respectively 85.7%(12/14) and 52.8%(19/36)，the positive expression rates of Ang-2 in RCC with hematuresis was higher than those without hematuresis，the differences were statistically significant(P<0.05).Conclusion：The expression of Ang-2 in the RCC is higher than those in the noncancerous renal tissue，which indicates that it may play an important role during the development of RCC.It has relationship to RCC angiogenesis， clinical stage and metastasis.
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【Key words】 Renal cell carcinoma； Angiopoietin-2； Immunohistochemical stain
First-author’s address：Shanxi Medical University，Taiyuan 030001，China
doi：10.3969/j.issn.1674-4985.2015.35.005
肾细胞癌(Renal cell carcinoma，RCC)是泌尿系统最常见的恶性肿瘤之一，又称肾腺癌，简称肾癌。在我国泌尿系统恶性肿瘤中发病率仅次于膀胱癌，约占成人恶性肿瘤的2%～3%[1]。RCC起源于肾实质泌尿小管上皮系统，是一类血管形成极为丰富的实性肿瘤，而新生血管形成在肿瘤的发生、发展及转移过程中起关键作用。近年来研究发现，促血管生成素(Angiopoietin，Ang)在肿瘤血管调控中起着重要作用。促血管生成素是血管内皮细胞的特异性酪氨酸激酶受体Tie-2的天然配体，目前发现的构型有4种，其中Ang-2是肿瘤性血管新生起始的强化因素，与肿瘤血管的生成关系密切[2]。本研究探讨Ang-2在肾细胞癌中的表达及其与患者性别、年龄、血尿及临床分期的关系。
1 资料与方法
1.1 一般资料 选取山西医科大学第一医院泌尿外科2013年1月-2014年12月间的50例肾癌手术切除标本，所有肾癌患者术前均未行放、化疗及免疫治疗，且均为单侧、单发，术后病理结果证实为肾细胞癌。其中男28例，女22例；年龄34～78岁，中位年龄61岁；术前有血尿者为14例，无血尿者为36例；根据美国癌症联合委员会(AJCC)肾癌临床分期标准分为：Ⅰ期20例，Ⅱ期17例，Ⅲ期9例，Ⅳ期4例。此外6例正常肾组织取自肾外伤及肾结石切除标本。, 百拇医药(杨文杰 刘尚莹 陈强 王杰)
【关键词】 肾细胞癌； 促血管生成素-2； 免疫组化
【Abstract】 Objective：To study the expression and significance of Ang-2 in renal cell carcinoma. Method：The expression of Ang-2 was measured by immunohistochemistry in tissue sections from 6 cases of normal renal tissues， 50 cases of RCC and their pericarcinous tissues，their correlation with clinical data features were analyzed.Result：The positive rate for expression of Ang-2 in RCC， their pericarcinous and normal renal tissue were respectively 62.0%(31/50)，28.0%(14/50) and 0(0/6)，the expression of Ang-2 in RCC was significantly higher than those in pericarcinous tissue and normal renal tissue，the difference was statistically significant among them(P<0.05).The positive expression rates of Ang-2 in the stages of Ⅰ-Ⅱ and Ⅲ-Ⅳ were respectively 51.4%(19/37) and 92.3%(12/13)，the positive expression rates of Ang-2 in the stages of Ⅲ-Ⅳ was higher than those in the stages of Ⅰ-Ⅱ；the positive expression rates of Ang-2 in patients with and without hematuresis were respectively 85.7%(12/14) and 52.8%(19/36)，the positive expression rates of Ang-2 in RCC with hematuresis was higher than those without hematuresis，the differences were statistically significant(P<0.05).Conclusion：The expression of Ang-2 in the RCC is higher than those in the noncancerous renal tissue，which indicates that it may play an important role during the development of RCC.It has relationship to RCC angiogenesis， clinical stage and metastasis.
, http://www.100md.com
【Key words】 Renal cell carcinoma； Angiopoietin-2； Immunohistochemical stain
First-author’s address：Shanxi Medical University，Taiyuan 030001，China
doi：10.3969/j.issn.1674-4985.2015.35.005
肾细胞癌(Renal cell carcinoma，RCC)是泌尿系统最常见的恶性肿瘤之一，又称肾腺癌，简称肾癌。在我国泌尿系统恶性肿瘤中发病率仅次于膀胱癌，约占成人恶性肿瘤的2%～3%[1]。RCC起源于肾实质泌尿小管上皮系统，是一类血管形成极为丰富的实性肿瘤，而新生血管形成在肿瘤的发生、发展及转移过程中起关键作用。近年来研究发现，促血管生成素(Angiopoietin，Ang)在肿瘤血管调控中起着重要作用。促血管生成素是血管内皮细胞的特异性酪氨酸激酶受体Tie-2的天然配体，目前发现的构型有4种，其中Ang-2是肿瘤性血管新生起始的强化因素，与肿瘤血管的生成关系密切[2]。本研究探讨Ang-2在肾细胞癌中的表达及其与患者性别、年龄、血尿及临床分期的关系。
1 资料与方法
1.1 一般资料 选取山西医科大学第一医院泌尿外科2013年1月-2014年12月间的50例肾癌手术切除标本，所有肾癌患者术前均未行放、化疗及免疫治疗，且均为单侧、单发，术后病理结果证实为肾细胞癌。其中男28例，女22例；年龄34～78岁，中位年龄61岁；术前有血尿者为14例，无血尿者为36例；根据美国癌症联合委员会(AJCC)肾癌临床分期标准分为：Ⅰ期20例，Ⅱ期17例，Ⅲ期9例，Ⅳ期4例。此外6例正常肾组织取自肾外伤及肾结石切除标本。, 百拇医药(杨文杰 刘尚莹 陈强 王杰)